Limited protection conferred by a DNA vaccine against a lethal pseudorabies virus infection at day 5 postvaccination.
نویسندگان
چکیده
No pseudorabies virus (PRV)-specific neutralizing or immunoglobulin G1-type antibodies were detected in sera 5 days after injection of a DNA vaccine against PRV infection in pigs. PRV-stimulated peripheral blood mononuclear cells produced gamma interferon mRNA in vitro. Two out of five pigs recovered from lethal PRV infection without attenuation of nasal viral excretion.
منابع مشابه
The Effect of Glycoprotein B DNA Immuniation on HSV-l Lethal Infection of BALB/c Mice
Background and Aims: Glycoprotein B (gB) is among the most important immunogenic proteins of HSV-1 that is now being used as recombinant vaccine to induce protectively against the virus. It is believed that gB is responsible for induction of neutralizing antibody and up to 90% of CTL (Cytotoxic T Cell) activity against HSV-1 in some haplotypes. Methods: In the present study the antibody titer ...
متن کاملDifferential segregation of protective immunity by encoded antigen in DNA vaccine against pseudorabies virus.
A murine model immunized with plasmid DNA vaccine expressing three glycoproteins pCIgB, pCIgC and pCIgD were used to examine the relative potency of major glycoproteins as well as the contribution of immunological parameters in providing protective immunity against the pseudorabies virus (PrV). Among the three glycoprotein-encoded plasmid DNA vaccines, pCIgB produced the strongest response of P...
متن کاملEfficacy of a Parainfluenza Virus 5 (PIV5)-Based H7N9 Vaccine in Mice and Guinea Pigs: Antibody Titer towards HA Was Not a Good Indicator for Protection
H7N9 has caused fatal infections in humans. A safe and effective vaccine is the best way to prevent large-scale outbreaks in the human population. Parainfluenza virus 5 (PIV5), an avirulent paramyxovirus, is a promising vaccine vector. In this work, we generated a recombinant PIV5 expressing the HA gene of H7N9 (PIV5-H7) and tested its efficacy against infection with influenza virus A/Anhui/1/2...
متن کاملPseudorabies Virus Variant in Bartha-K61–Vaccinated Pigs, China, 2012
The widely used pseudorabies virus (PRV) Bartha-K61 vaccine has played a key role in the eradication of PRV. Since late 2011, however, a disease characterized by neurologic symptoms and a high number of deaths among newborn piglets has occurred among Bartha-K61-vaccinated pigs on many farms in China. Clinical samples from pigs on 15 farms in 6 provinces were examined. The PRV gE gene was detect...
متن کاملA single immunization with HA DNA vaccine by electroporation induces early protection against H5N1 avian influenza virus challenge in mice
BACKGROUND Developing vaccines for the prevention of human infection by H5N1 influenza viruses is an urgent task. DNA vaccines are a novel alternative to conventional vaccines and should contribute to the prophylaxis of emerging H5N1 virus. In this study, we assessed whether a single immunization with plasmid DNA expressing H5N1 hemagglutinin (HA) could provide early protection against lethal c...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Clinical and vaccine immunology : CVI
دوره 14 4 شماره
صفحات -
تاریخ انتشار 2007